The pulled-down fragments are then analyzed by high-throughput sequencing. This enriches the sample for informative, hybrid molecules on a genome-wide scale. The crosslinks are reversed, and the library is then fragmented, and the biotinylated ligation junctions are pulled-down by streptavidin beads. Namely, which biotinylated nucleotides are incorporated into the restriction overhangs before blunt-end ligation is carried out. Hi-C protocols require modification to the 3C library preparation. It can capture the spatial interaction information between different gene loci in the whole genome, obtain three-dimensional (3D) structure information of chromatin and study the DNA elements regulating genes in the 3D space. Hi-C is the genome-wide extension of 3C, which refers to the capture of chromosome conformation based on high-throughput technique. At Creative BioMart, we will provide you with the best Hi-C sequencing service to achieve your research goals. Hi-C can be combined with RNA-Seq, ChIP-Seq, ATAC-Seq and other data for joint analysis, so as to elaborate the relevant mechanisms of the formation of biological traits from the perspective of gene regulatory networks and epigenetic networks. The high-through chromosome conformation capture (Hi-C) technique extends the chromosome conformation capture (3C) technique to map chromatin contacts across the genome, and it has been applied to the study of in situ chromatin interactions.
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